Cellulase Immobilization on Nano-carriers for Reuse in Cellulose Hydrolysis

Project objectives are as follows: (1) Develop methods to immobilize cellulases on magnetically separable nano-carriers. (2) Investigate the activities of immobilized cellulases in cellulose hydrolysis. (3) Evaluate the reusability of immobilized cellulases in cellulose hydrolysis. The proposed research combines the hydrophilic cellulase conjugation with the magnetic cellulase separation, which offers the dual benefits of cellulase activity retention and efficient cellulase recovery. The project team expects the cellulase recovery with activity retained to greatly lower the enzyme cost and improve the enzyme conversion efficiency in cellulosic biofuel production. Expected outcomes are as follows: (1) Development of immobilized cellulases for cellulose hydrolysis. (2) Magnetic separation of cellulases for reuse in cellulose hydrolysis. (3) Understanding of cellulase activity and reusability in cellulose hydrolysis.

• Supplemental Notes:
  • Program Information: Advanced Research, Biobased Research

Language

• English

Project

• Status: Completed
• Funding: $70000.00
• Sponsor Organizations:

  Research and Innovative Technology Administration

  Department of Transportation
  1200 New Jersey Avenue, SE
  Washington, DC  United States  20590
• Project Managers:

  Johnson, Shawn

• Performing Organizations:

  Oklahoma State University, Stillwater

  Stillwater, OK  United States  74078
• Principal Investigators:

  Wilkins, Mark

  Mao, Yu

• Start Date: 20091201
• Expected Completion Date: 0
• Actual Completion Date: 20121130
• Source Data: RiP Project 31382

Subject/Index Terms

• TRT Terms: Biomass fuels; Cellulose; Hydrolysis; Renewable energy sources
• Uncontrolled Terms: Immobilization; Reuse
• Subject Areas: Energy; Transportation (General);

Filing Info

• Accession Number: 01572202
• Record Type: Research project
• Source Agency: Department of Transportation
• Files: RIP
• Created Date: Aug 4 2015 1:00AM