Plant Pathogens of Feedstock Crops as a Source of Useful Enzymes

Plant pathogenic fungi are a rich and untapped source of cell wall degrading enzymes for feedstock conversion. Switchgrass pathogens are ideal candidates to prospect for enzymes usable in conversion of switchgrass biomass because these fungal strains have evolved the capacity to degrade cell walls of this species. Therefore this project will target biopropecting at necrotrophic fungal pathogens isolated from switchgrass and other potential feedstock crops in the Northeast. The ultimate long-term goal is to identify and develop enzyme systems for optimal bioethanol production. The end products should be higher catalytic function enzymes that can be used at lower amounts, at reduced overall costs and with higher efficiency in lignocellulosic bioconversion. Expected Outcomes: The investigators expect to identify superior fungal isolates that produce cellulases and xylanses equivalent to or better than the industry standard Trichoderma reesii. Symptomatic tissues of switchgrass and other biofeedstock species will be collected from natural and cultivated stands of these species in the field and plant pathogenic fungi will be isolated from these tissues, cultured, identified, and archived. The project will build a collection of 400 isolates of diverse fungal genera and species. These fungal isolates will be screened for growth on lignocellulosic substrates and for cellulase and xylanase activities.

• Supplemental Notes:
  • Program Information: Advanced Research, Biobased Research

Language

• English

Project

• Status: Active
• Funding: $100000.00
• Sponsor Organizations:

  Research and Innovative Technology Administration

  Office of Research, Development, and Technology
  1200 New Jersey Avenue, SE
  Washington, DC    20590
• Project Managers:

  Johnson, Shawn

• Performing Organizations:

  Cornell University

  Ithaca, NY  United States  14853
• Principal Investigators:

  Bergstrom, Gary

• Start Date: 20080701
• Expected Completion Date: 0
• Actual Completion Date: 0
• Source Data: RiP Project 31361

Subject/Index Terms

• TRT Terms: Biomass; Crops
• Uncontrolled Terms: Bioethanol; Enzymes; Feedstock; Pathogens
• Subject Areas: Energy; Transportation (General); I15: Environment;

Filing Info

• Accession Number: 01467541
• Record Type: Research project
• Source Agency: Department of Transportation
• Files: RIP, USDOT
• Created Date: Jan 3 2013 3:35PM